The Technology

Beckman Coulter Biomek FX systems

The FGC has two Dual arm Biomek FX liquid handling automation systems. The primary system is equipped with both a Liconic STX-44 live cell incubator and a large capacity Cytomat ambient storage hotel. The Liconic STX-44 incubator has temperature control, humidity control, CO2 control and can shake up to 1200 RPM. This incubator is capable of meeting the needs of most cell types for live cell screening. This system is also equipped with a detachable compound pinning tool, sonicating wash station, and pin drying fan. This robot can perform precision pipetting down to 0.2 µl and precision pinning down to 10 nl.

The second Dual arm Biomek FX liquid handling automation system is equipped with both a large capacity incubated Cytomat storage hotel and and a large capacity Cytomat ambient storage hotel. This system is configured to perform a wide array of liquid handling operations. Both systems can perform high-throughput cherry-picking and plate refactoring, dilution and normalization, and the automation of most molecular biology protocols.

FGC Machine

Operetta CLS - High-content Analysis Microscope Imaging System

The Operetta CLS high-content analysis system has all the speed, sensitivity and resolution you need to characterize fine sub-cellular details . The Operetta is designed for fully-automated high-capacity, quantitative cellular analysis of fixed or live cells. It contains the highly sensitive sCMOS camera that provides a large field of view and high resolution image capture. The Operetta's Harmony software lets you find even subtle phenotypic changes.

Operetta CLS Machine

Objectives

  • 5x Air Objective: NA 0.16, WD 12.1 mm (field of view: approx. 2583 μm x 2583 μm)
  • 10x Air Objective: NA 0.3, WD 5.2 mm (field of view: approx. 1292 μm x1292 μm)
  • 20x Air Objective: NA 0.4, WD 8.28 mm (field of view: approx. 646 μm x 646 μm)
  • 20x Water Immersion Objective: NA 1.0, WD 1.7 mm (field of view: approx. 646 μm x 646 μm)
  • 40x Water Immersion Objective: NA 1.1, WD 0.62 mm (field of view: approx. 323 μm x 323 μm)
  • 63x Water Immersion Objective: NA 1.15, WD 0.6 mm (field of view: approx. 205 μm x 205 μm)
  • 4.7Mpx sCMOS camera (2160x2160), 16 bit resolution, 6.5um pixel size
  • Motorized 6 position objective turret with 3 air and 3 water immersion objectives
  • High resolution, linear drive scanning stage
  • High speed precision laser autofocus
  • Transmitted light unit (LED: 740nm) for Brightfield and Digital Phase Contrast imaging
  • Emission filters:

    • 430-500 nm

    • 470-515 nm

    • 500-550 nm

    • 525-580 nm

    • 570-650 nm

    • 655-760 nm

  • Spinning Disk enables optical sectioning
  • Confocal imaging improves optical resolution and contrast
  • Pinhole disk reduces out-of-focus light
  • LED light engine equipped with 8 high power LEDs with the following excitation wavelengths:
    • 355-385 nm
    • 390-420 nm
    • 435-460 nm
    • 460-490 nm
    • 490-515 nm
    • 530-560 nm
    • 615-645 nm
    • 650-675 nm
  • Environmental Controls for live cell experiments:
    • Temperature can be controlled between 37°C - 42°C
    • CO2 concentration can be controlled between 1-10%

Thermo Scientific Multidrop

The Thermo Scientific Multidrop is a versatile liquid handling system designed for use in a variety of laboratory applications. It is capable of accurately and reliably dispensing a wide range of liquid volumes, from nanoliters to microliters, with precision and reproducibility. The Multidrop is commonly used for DNA and protein sample preparation, cell culture and PCR setup, serial dilutions, and other liquid handling tasks. It can also be used for sample preparation for genotyping and sequencing, and for creating standard curves for quantitative assays. The Multidrop is also suitable for use in drug discovery, high-throughput screening, and other research applications. Its intuitive software and user-friendly interface allow for quick and easy setup and operation.

Thermo Scientific Multidrop