Myron K Jacobson, PhD
Professor
Department:
Pharmacology and Toxicology
(520) 626-5957
Fax:
(520) 626-8567
E-mail:
Location:
Cancer Center 3985B
Bio / Research:
To maintain the genomic integrity of the organism, individual cells within the organism must make appropriate life and death decisions. Ongoing research is aimed at understanding the molecular mechanisms by which cells respond to toxic chemicals and radiation by activating pathways that lead to repair of damage and cell recovery or to cell death by apoptosis or necrosis. The information gained is used toward the design of therapeutic strategies for the treatment of diseases in which cells are either inappropriately resistant to cell death (cancer) or inappropriately sensitive to cell death (heart attack, stroke, etc.).
The laboratory is studying the involvement of poly(ADP-ribose) polymerases (PARPs) and poly(ADP-ribose) glycohydrolase (PARG) in the responses of cells to potentially toxic conditions. PARPs and PARG catalyze the opposing arms of ADP-ribose polymer cycles involved in alternative responses to nuclear damage. These include the repair of nuclear DNA and protein damage, pathways leading to programmed cell death (apoptosis), and pathways leading to cell death by necrosis. ADP-ribose polymer cycles are also involved in the maintenance of the telomere structures that protect the ends of chromosomes.
The laboratory uses a structure-based approach for characterization of its molecular targets. Techniques routinely used in the laboratory include protein isolation and microsequencing, cDNA cloning, heterologous protein expression and characterization, site-directed mutagenesis, expression and characterization of dominant negative proteins in cultured cells, and the use of transgenic animals.
Education:BS with Honors, University of Wisconsin, Platteville, 1965, Chemistry
PhD, Kansas State University, Manhattan, 1969, Biochemistry
Postdoctoral Training
1969-1971, University of Utah, Salt Lake City with Karl G. Lark
1972-1973, Mayo Clinic and Foundation, Rochester, Minnesota with Carl Bernofsky
Honors and Awards:
2008 Joseph B. Swintosky Distinguished Lecturer, University of Kentucky
2007 Bioscience Company of the Year,
2007 Alumni Fellow, College of Arts & Sciences, Kansas State University
2006 Outstanding University Achievement Award, University of Arizona
2005 The E. Bruce Street, Sr./Roger J. Williams Award in Preventive Nutrition, University of North Texas Health Science Center, Fort Worth
2003 Foundation for Promotion of Cancer Research Fellowship, National Cancer Center Research Institute, Tokyo, Japan
2002 Rho Chi Distinguished Lecturer, University of Kentucky College of Pharmacy
1997 Outstanding Instructor for Professional Year 1, UK College of Pharmacy
1992 M.L. Coleman Award for Excellence in Teaching UNTHSC-FW
1991 M.L. Coleman Award for Excellence in Teaching UNTHSC-FW
1991 Distinguished Alumnus Award, University of Wisconsin, Platteville
1988 M.L. Coleman Award for Excellence in Teaching UNTHSC-FW
1986 Outstanding Teacher in the Pre-Clinical Sciences
1985 Pre-Clinical Sciences Teaching Professor of the Year
1983 The Alexander von Humboldt Foundation Research Fellowship
1970 National Institutes of Health Postdoctoral Research Fellowship
1969 Phi Lambda Upsilon Graduate Research Award
1966 National Aeronautics and Space Administration Predoctoral Fellowship
1965 Honor Graduate, University of Wisconsin, Platteville
Jacobson, E.L., Kim, H., Kim, M. and Jacobson, M.K. Niacin: Vitamin and Antidyslipidemic Drug. (2008) In: Water-Soluble Vitamins: Subcellular Biochemistry, Stanger, O. (ed), Springer-Verlag publishers, Berlin, Heidelberg, New York, in press.
Williams, J.D., Jacobson, E.L., Kim, H., Kim, M. and Jacobson, M.K. Folate in Skin Cancer Prevention. (2008) In: Water-Soluble Vitamins: Subcellular Biochemistry, Stanger, O. (ed), Springer-Verlag publishers, Berlin, Heidelberg, New York, in press.
Tashtoush, B.M., Jacobson, E.L. and Jacobson, M.K. Validation of a Simple and Rapid HPLC Method for Determination of Metronidazole in Dermatological Formulations. (2008) Drug Development and Industrial Pharmacy, 34(8), 840-844.
Tashtoush, B.M., Qandil, A.M., Jacobson, E.L. and Jacobson, M.K. Analysis and Stability Study of Octyl Nicotinate in Aqueous Solutions and Skin Homogenate by LC. (2008) Chromatographia, 68(1-2), 135-138.
Tashtoush, B.M., Jacobson, E.L. and Jacobson, M.K. UVA is the Major Contributor to the Photodegradation of Tretinoin and Isotretinoin: Implications for Development of Improved Pharmaceutical Formulations. (2008) International Journal of Pharmaceutics, 352(1-2), 123-128.
Jacobson, M.K., Kim, H., Coyle, W.R., Kim, M., Coyle, D.L., Rizer, R.L. and Jacobson E.L. Effect of Myristyl Nicotinate on Retinoic Acid Therapy for Facial Photodamage. (2007) Experiment Dermatology, 16(11), 927-35.
Tashtoush B.M., Kim, H., Jacobson, E.L. and Jacobson, M.K. Formulation Compatibility of Myristyl Nicotinate with Drugs Used to Treat Dermatological Conditions Involving an Impaired Skin Barrier. (2007) Drug Development and Industrial Pharmacy, 33(11), 1176-82.
Meyer, R.G., Meyer-Ficca, M.L., Whatcott, C.J., Jacobson, E.L. and Jacobson, M.K. Two Small Enzyme Isoforms Mediate Mammalian Mitochondrial Poly(ADP-ribose) Glycohydrolase (PARG) Activity. (2007) Experimental Cell Research, 313(13), 2920-36.
Gao, H., Coyle, D.L., Meyer-Ficca, M.L., Meyer, R.G., Jacobson, E.L., Wang, Z-Q and Jacobson, M.K. Altered Poly(ADP-ribose) Metabolism Impairs Cellular Responses to Genotoxic Stress in a Hypomorphic Mutant of Poly(ADP-ribose) Glycohydrolase. (2007) Experimental Cell Research, 313(5), 984-996.
Jacobson, E.L. Kim, H., Kim, M., Williams, J.D., Coyle, D.L., Coyle, W.R., Grove, G., Rizer, R.L., Stratton, M.S. and Jacobson, M.K. A Topical Lipophilic Niacin Derivative increases NAD, Epidermal Differentiation and Barrier Function in Photodamaged Skin. (2007) Experimental Dermatology, 16(6), 490-499.
Tashtoush, B.M., Qasem, J., Williams, J.D., DeWald, T.P., Jacobson, E.L. and Jacobson M.K. Analysis and Stability Study of Myristyl Nicotinate in Dermatological Preparations by High-Performance Liquid Chromatography. (2007) Journal of Pharmaceutical and Biomedical Analysis, 43(3), 893-899.
Tashtoush, B.M., Jacobson, E.L. and Jacobson, M.K. A Rapid HPLC Method for Simultaneous Determination of Tretinoin and Isotretinoin in Dermatological Formulations. (2007) Journal of Pharmaceutical and Biomedical Analysis, 43(3), 859-864.
Wondrak, G.T., Jacobson, M.K. and Jacobson, E.L. Antimelanoma Activity of Apoptogenic Carbonyl Scavengers. Journal of Pharmacology and Experimental Therapeutics, 316, 805-814, 2006.
Wondrak, G.T., Jacobson, M.K. and Jacobson, E.L. Endogenous UVA-Photosensitizers: Mediators of Skin Photodamage and Novel Targets for Skin Photoprotection. (2006) Photochemical and Photobiological Sciences, 5, 215-237. Selected for inclusion in the RSC’s Chemical Biology Virtual Journal.
Draelos, Z.D., Jacobson, E.L., Kim, H., Kim, M. and Jacobson, M.K. A Pilot Study Evaluating the Efficacy of Topically Applied Niacin Derivatives for Treatment of Female Pattern Alopecia. Journal of Cosmetic Dermatology, 4, 258-261, 2005.
Catz, P., Shinn, W., Kapetanovic, I.M., Kim, H., Kim, M., Jacobson, E.L., Jacobson, M.K. and Green, C.E. Simultaneous Determination of Myristyl Nicotinate, Nicotinic Acid, and Nicotinamide in Rabbit Plasma by Liquid Chromatography—Tandem Mass Spectrometry Using Methyl Ethyl Ketone as a Deproteinization Solvent. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 829, 123-135, 2005.
Wondrak, G.T., Jacobson, M.K. and Jacobson, E.L. An Emerging Molecular Target in Melanoma: Cellular Carbonyl Stress and the Inhibition of Mitochondrial Survival Pathways by Carbonyl Scavenger Agents. (2005) Current Cancer Therapy Reviews, 1, 271-276.
Jacobson, E.L., Kim, H., Kim, M., Wondrak, G.T. and Jacobson, M.K. Developing Topical Prodrugs for Skin Cancer Prevention, (2005) In Fundamentals of Cancer Prevention, D. Alberts and L. Hess, Eds., Springer-Verlag Publishers, Berlin, Heidelberg, New York, pp. 139-160.
Patel, C.N., Koh, D.W., Jacobson, M.K. and Oliveira, M.A. Identification of Three Critical Acidic Residues of Poly(ADP-ribose) Glycohydrolase Involved in Catalysis: Determining the PARG Catalytic Domain. (2005) Biochemical Journal, 388, 493-500.
Cervantes-Laurean, D., Roberts, M.J., Jacobson, E.L. and Jacobson, M.K. Nuclear Proteasome Activation and Degradation of Carboxymethylated Histones in Human Keratinocytes Following Glyoxal Treatment. (2005) Free Radical Biology and Medicine, 38, 786-795.
Meyer-Ficca, M.L., Meyer, R.G., Jacobson, E.L. and Jacobson, M.K. Poly(ADP-ribose) polymerases: Managing Genome Stability. (2005) International Journal of Biochemistry and Cell Biology, 37, 920-926.
Wondrak, G.T., Jacobson, M.K. and Jacobson, E.L. Identification of Quenchers of Photoexcited States as Novel Agents for Skin Photoprotection. (2005) Journal of Pharmacology and Experimental Therapeutics, 312, 482-91.
Meyer, R.G., Meyer-Ficca, M.L., Jacobson, E.L. and Jacobson, M.K., Enzymes in Poly(ADP-Ribose) Metabolism, (2004) In Poly(ADP-Ribosyl)ation, Alexander Bürkle, Ed. Landes Bioscience/Eurekah.com, Georgetown, TX, pp. 1-12.
Kim, H., Jacobson, E.L., Jacobson, M.K. and Lacko, A.G. ApoA-1 Milano and Regression of Atherosclerosis. (2004) JAMA Letters, 291, 1319.
Chang, P., Jacobson, M.K. and Mitchison, T.J. Poly(ADP-ribose) is Required for Spindle Assembly and Structure. (2004) Nature, 432, 645-649.
Cortes, U., Tong, W.-M., Coyle, D.L., Meyer-Ficca, M.L., Meyer, R.G., Petrilli, V., Herceg, Z., Jacobson, E.L., Jacobson, M.K. and Wang, Z.-Q. Depletion of the 110 kDa Isoform of Poly(ADP-ribose) Glycohydrolase (PARG) Increases Sensitivity to Genotoxic and Endotoxic Stress in Mice. (2004) Molecular and Cellular Biology, 24, 7163-7178.
Wondrak, G.T., Roberts, M.J., Jacobson, M.K. and Jacobson, E.L. 3-Hydroxypyridine Chromophores are Endogenous Sensitizers of Photooxidative Stress in Human Skin Cells. (2004) Journal of Biological Chemistry, 279, 30009-30020.
Meyer-Ficca, M.L., Meyer, R.G., Coyle, D.L., Jacobson, E.L. and Jacobson, M.K. Human poly(ADP-ribose) glycohydrolase is expressed in alternative splice variants yielding isoforms that localize to different cell compartments. (2004) Experimental Cell Research, 297, 521-532.