Yin Chen, PhD

Associate Professor
Member
Southwest Environmental Health Sciences Center
Member
BIO5
Department(s): 
Pharmacology & Toxicology

ychen@pharmacy.arizona.edu
520-626-4715
FAX: 520-626-2466
Pharmacy (Skaggs) Building 232

Research

Airway epithelial differentiation and mucous cell metaplasia in chronic airway diseases
Mucous cell metaplasia and mucus over-production are hallmarks of almost all chronic airway diseases (e.g. asthma, chronic obstructive pulmonary diseases, cystic fibrosis, etc.) and significantly increase the morbidity and mortality. No therapy is available besides the mechanical suction. We are now investigating the function and regulation of mucin genes in asthma pathogenesis using both in vivo (gene targeting model, induced mouse model of asthma, as well as tissues and secretions from asthmatic patients) and in vitro (differentiated epithelial culture and cell line) models.

A new paradigm in virus-induced asthma exacerbation
Airway rhinovirus (RV) infection is the major cause of asthma exacerbation, a severe precipitation of the symptom in otherwise stable asthmatics who are often still under the routine medication. Thus, asthma exacerbation may have a different pathogenic mechanism that is largely unknown at present. Among different asthma-inducing allergens, Alternaria (Alt) is a fungal species that causes asthma in arid and semi-arid areas. In collaboration with the researchers in Arizona Respiratory Center, we have found the shift of airway response to viral infection during Alt exposure, which promotes inflammation and depresses antiviral response. This shift causes further increase of viral production and inflammation, similar to what would happen in the airways of asthma exacerbation. We are currently investigating the potential underlying mechanisms using both in vivo and in vitro models.
 

Education: 

BS, Biochemistry, 1996,  Nanjing University, Nanjing, Jiangsu, China
PhD, Genetics, 2001, University of California, Davis
 

Publications

  1. Lingxiang Zhu, Pakkei Lee, Dongfang Yu, Shasha Tao, Yin Chen. 2010. Cloning and characterization of human MUC19 gene. American Journal of Respiratory Cell and Molecular Biology. Epub ahead of print.
  2. Fujisawa T, Chang MM, Velichko S, Thai P, Hung LY, Huang F, Phuong N, Chen Y, Wu R. 2010. NF-{kappa}B Mediates IL-1ss- and IL-17A-induced MUC5B Expression in the Airway Epithelial Cells. American Journal of Respiratory Cell and Molecular Biology. Epub ahead of print
  3. Lingxiang Zhu, Pak-kei Lee, Wai-ming Lee, Yuhua Zhao, Dongfang Yu and Yin Chen. 2009. Rhinovirus induced major airway mucin production involves a novel TLR3-EGFR dependent pathway. American Journal of Respiratory Cell and Molecular Biology. American Journal of Respiratory Cell and Molecular Biology. 40(5):610-9.
  4. Lingxiang Zhu, Jingbo Pi, Shinichiro Wachi, Melvin E. Andersen, Reen Wu and Yin Chen. 2008. Identification of Nrf2-dependent airway epithelial adaptive response to pro-inflammatory oxidant-hypochlorous acid (HOCl) challenge by transcription profiling. American Journal of physiology-Lung Cellular and Molecular Physiology. 294(3):L469-77.
  5. Yu DF, Chen Y, Han JM, Zhang H, Chen XP, Zou WJ, Liang LY, Xu CC, and Liu ZG. 2007. MUC19 Expression in Human Ocular Surface and Lacrimal Gland and its Alteration in Sjögren Syndrome Patients. Experimental Eye Research. Epub ahead of print.
  6. Wu DY, Wu R, Chen Y, Tarasova N, Chang MM. 2007. PMA Stimulates MUC5B Gene Expression through a Sp1-based Mechanism in Airway Epithelial Cells. American Journal of Respiratory Cell and Molecular Biology. Jun. 28. Epub ahead of print.
  7. Chen Y, Hamati E, Lee W-M, Wachi S, Schnurr D, Shigeo Y, Dolganov G, Boushey H, Avila P, Wu R 2006. Rhinovirus Induces Airway Epithelial Gene Expression through dsRNA and Interferon-dependent Pathways. American Journal of Respiratory Cell and Molecular Biology. 34(2):192-203
  8. Thai P, Chen Y, Dolganov G, and Wu R 2005. Differential Regulation of MUC5AC/ Muc5ac and hCLCA-1/Gob-5 Expression in Airway Epithelium. American Journal of Respiratory Cell and Molecular Biology. 33(6):523-30.
  9. Kao CY, Huang F, Chen Y, Thai P, Wachi S, Kim C, Tam L and Wu R. 2005. Up-regulation of CC Chemokine Ligand 20 Expression in Human Airway Epithelium by IL-17 through a JAK-Independent but MEK/NF-kappaB-Dependent Signaling Pathway. Journal of Immunology. 175(10):6676-85
  10. Harper R, Xu C, Eiserich JP, Chen Y, Kao CY, Thai P, Setiadi H, Wu R. 2005. Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium. FEBS Lett. 579(21): 4911-4917.
  11. Xu C, Di P, Chen Y, Privalsky M, Wu R., and Harper R. 2004. Identification of a Novel MAGE D2 Antisense RNA Transcript in Human Tissues. Biochemical and Biophysical Research Communications. 324(1):199-204.
  12. Kao, C.-Y. R., Y. Chen, P. Thai, S. Wachi, F. Huang, C. Kim, R. W. Harper, and R. Wu. 2004. Interleukin-17 markedly up-regulates β-defensin 2 expression in human airway epithelium via JAK and NF-κB pathways. Journal of Immunology. 173(5):3482-91
  13. Chen Y, Zhao YH, Kalaslavadi TB, Hamati E, Nehrke K, Le AD, Ann DK, Wu R. 2004. Genome-Wide Search and Identification of a Novel Gel-Forming Mucin MUC19/Muc19 in Glandular Tissues. American Journal of Respiratory Cell and Molecular Biology. 30(2): 155-165.
  14. Chen Y, Thai P, Zhao YH, Ho YS, DeSouza MM, and R. Wu. 2003. Stimulation of Airway Mucin Gene Expression by IL-17 through IL-6 paracrine/autocrine loop. Journal of Biological Chemistry. 278(19):17036-17043.
  15. Kao CY, Chen Y, Zhao YH, and R. Wu. 2003. ORFeome Based Search of Airway Epithelial Cell-Specific Novel Human {beta}-Defensin Genes. American Journal of Respiratory Cell and Molecular Biology. 29(1):71-80.
  16. Chen, Y., Y. Zhao, Y.P. Di, and R. Wu. 2001. Characterization of Human MUC5B Gene Expression in Airway Epithelium and the Genomic Clone of the Amino-Terminal and 5'-Flanking Region. American Journal of Respiratory Cell and Molecular Biology 25(5): 542-53.
  17. Chen, Y., Y. Zhao, R. Wu. 2001. In silico Cloning of Mouse Muc5b Gene and Upregulation of Its Gene Expression in Mouse Asthma Model. American Journal of Respiratory and Critical Care Medicine 164(6): 1059-66.
  18. Chen, Y., Y. Zhao, and R. Wu. 2001. Differential Regulation of Airway Mucin Gene Expression and Mucin Secretion by Extracellular Nucleotide Triphosphates. American Journal of Respiratory Cell and Molecular Biology 25(4): 409-17.
  19. Yoneda K., K. Peck, M. M. J. Cang, K. Chimel, Y-P. Sher, J. Chen, P-C. Yang, Y. Chen, and R. Wu. 2001. Development of High-density DNA Microarray Membrane for Profiling Smoke- and Hydrogen Peroxide-induced Genes in a Human Bronchial Epithelial Cell Line. American Journal of Respiratory and Critical Care Medicine 164: 85S-89S.
  20. Deng, J., Y. Chen, and R. Wu. 2000. Induction of cell cornification and enhanced squamous-cell marker SPRR1 gene expression by phorbol ester are regulated by different signaling pathways in human conducting airway epithelial cells. American Journal of Respiratory Cell and Molecular Biology 22(5):597-603
     
Publications
Originally posted: September 9, 2013
Last updated: September 3, 2015
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